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ampl43 plasmid  (Addgene inc)


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    Addgene inc ampl43 plasmid
    Ampl43 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampl43 plasmid/product/Addgene inc
    Average 93 stars, based on 4 article reviews
    ampl43 plasmid - by Bioz Stars, 2026-05
    93/100 stars

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    ampl43 plasmid  (Addgene inc)
    93
    Addgene inc ampl43 plasmid
    Ampl43 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampl43 plasmid/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    ampl43 plasmid - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    ampl43  (Addgene inc)
    93
    Addgene inc ampl43
    a Schematic of <t>amPL43</t> expression vector for inducible CRISPRi library in S. cerevisiae . b The expression fold change of each target: ERG25 (three replicates), ERG11 (five replicates), and Sec14 (four replicates), as a result of presence of sgRNA, without induction after 24 hours, as measured by qPCR. The mean for each sample is represented by a solid line. c The expression fold change of each target: ERG25 (three replicates), ERG11 (three replicates), and Sec14 (two replicates), as a result of gRNA induction by ATc, was calculated over time by qPCR. The mean for each sample is represented by a solid line. d Schematic depicting the genomic region of PTA1 and ERV46. The gRNAs targeting the region between the two genes, depending on their proximity to each gene, could affect both genes. e Histogram depicting the number of gRNAs per gene in two library replicates. The dashed blue line denotes the median: ~6. f Scatter plot depicting the frequency of reads per gRNA between select biological replicates of the CRISPRi library. Pearson Correlation R value is reported for each pair.
    Ampl43, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampl43/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    ampl43 - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    ampl43  (Qiagen)
    90
    Qiagen ampl43
    a) Schematic of <t>amPL43</t> expression vector for inducible CRISPRi library in S. cerevisiae . gRNA scaffold region contains a NotI site for cloning gRNA sequences, an RPR1 promoter with a TetO site, and a constant region for the gRNA. b) The expression fold-change of each target: ERG25 (three replicates), ERG11 (three replicates), and Sec14 (two replicates), as a result of gRNA induction by ATc, was calculated over time by qPCR. The mean for each sample is represented by a solid line. c) Schematic depicting the genomic region of PTA1 and ERV46. The gRNAs targeting the region between the two genes, depending on their proximity to each gene, could affect both genes. d) Histogram depicting the number of gRNAs per gene in two library replicates. The dashed blue line denotes the median: ~6. e) Scatter plot depicting the frequency of reads per gRNA between select biological replicates of the CRISPRi library. Pearson Correlation R value is reported for each pair.
    Ampl43, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampl43/product/Qiagen
    Average 90 stars, based on 1 article reviews
    ampl43 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

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    a Schematic of amPL43 expression vector for inducible CRISPRi library in S. cerevisiae . b The expression fold change of each target: ERG25 (three replicates), ERG11 (five replicates), and Sec14 (four replicates), as a result of presence of sgRNA, without induction after 24 hours, as measured by qPCR. The mean for each sample is represented by a solid line. c The expression fold change of each target: ERG25 (three replicates), ERG11 (three replicates), and Sec14 (two replicates), as a result of gRNA induction by ATc, was calculated over time by qPCR. The mean for each sample is represented by a solid line. d Schematic depicting the genomic region of PTA1 and ERV46. The gRNAs targeting the region between the two genes, depending on their proximity to each gene, could affect both genes. e Histogram depicting the number of gRNAs per gene in two library replicates. The dashed blue line denotes the median: ~6. f Scatter plot depicting the frequency of reads per gRNA between select biological replicates of the CRISPRi library. Pearson Correlation R value is reported for each pair.

    Journal: Communications Biology

    Article Title: An inducible CRISPR interference library for genetic interrogation of Saccharomyces cerevisiae biology

    doi: 10.1038/s42003-020-01452-9

    Figure Lengend Snippet: a Schematic of amPL43 expression vector for inducible CRISPRi library in S. cerevisiae . b The expression fold change of each target: ERG25 (three replicates), ERG11 (five replicates), and Sec14 (four replicates), as a result of presence of sgRNA, without induction after 24 hours, as measured by qPCR. The mean for each sample is represented by a solid line. c The expression fold change of each target: ERG25 (three replicates), ERG11 (three replicates), and Sec14 (two replicates), as a result of gRNA induction by ATc, was calculated over time by qPCR. The mean for each sample is represented by a solid line. d Schematic depicting the genomic region of PTA1 and ERV46. The gRNAs targeting the region between the two genes, depending on their proximity to each gene, could affect both genes. e Histogram depicting the number of gRNAs per gene in two library replicates. The dashed blue line denotes the median: ~6. f Scatter plot depicting the frequency of reads per gRNA between select biological replicates of the CRISPRi library. Pearson Correlation R value is reported for each pair.

    Article Snippet: AmPl43 was also deposited to Addgene (Addgene ID: 161830).

    Techniques: Expressing, Plasmid Preparation

    a) Schematic of amPL43 expression vector for inducible CRISPRi library in S. cerevisiae . gRNA scaffold region contains a NotI site for cloning gRNA sequences, an RPR1 promoter with a TetO site, and a constant region for the gRNA. b) The expression fold-change of each target: ERG25 (three replicates), ERG11 (three replicates), and Sec14 (two replicates), as a result of gRNA induction by ATc, was calculated over time by qPCR. The mean for each sample is represented by a solid line. c) Schematic depicting the genomic region of PTA1 and ERV46. The gRNAs targeting the region between the two genes, depending on their proximity to each gene, could affect both genes. d) Histogram depicting the number of gRNAs per gene in two library replicates. The dashed blue line denotes the median: ~6. e) Scatter plot depicting the frequency of reads per gRNA between select biological replicates of the CRISPRi library. Pearson Correlation R value is reported for each pair.

    Journal: bioRxiv

    Article Title: An inducible CRISPR-interference library for genetic interrogation of Saccharomyces cerevisiae biology

    doi: 10.1101/2020.03.05.978619

    Figure Lengend Snippet: a) Schematic of amPL43 expression vector for inducible CRISPRi library in S. cerevisiae . gRNA scaffold region contains a NotI site for cloning gRNA sequences, an RPR1 promoter with a TetO site, and a constant region for the gRNA. b) The expression fold-change of each target: ERG25 (three replicates), ERG11 (three replicates), and Sec14 (two replicates), as a result of gRNA induction by ATc, was calculated over time by qPCR. The mean for each sample is represented by a solid line. c) Schematic depicting the genomic region of PTA1 and ERV46. The gRNAs targeting the region between the two genes, depending on their proximity to each gene, could affect both genes. d) Histogram depicting the number of gRNAs per gene in two library replicates. The dashed blue line denotes the median: ~6. e) Scatter plot depicting the frequency of reads per gRNA between select biological replicates of the CRISPRi library. Pearson Correlation R value is reported for each pair.

    Article Snippet: To generate the library, amPL43 was maxiprepped (Qiagen, Hilden, Germany) and cut with NotI.

    Techniques: Expressing, Plasmid Preparation, Clone Assay